My Ethical Theory Essay Example | Topics and Well Written Essays - 750 words - 1

My Ethical Theory - Essay Example Consequentialist ethics â€Å"holds that the moral rightness of acts, which holds that whether an act is morally right, depends only on the consequences of that act or of something related to that act, such as the motive behind the act or a general rule requiring acts of the same kind† (Standford Encyclopedia of Philosophy 2011 a). This is not utilitarianism which â€Å"generally held to be the view that the morally right action is the action that produces the most good† (Stanford Encyclopedia of Philosophy b 2011). It is not also virtue ethics because it does not mean to do just for the sake of being good. Considering the potency of the drug curing many social ills and making people happy, it would be dangerous to keep It secret. One way or another It will leak out and when it does, It will be blown out of proportion. Worst, if It falls into the wrong hands, it would be misused and will only be used for profit and personal gain. The only benefit of keeping the drug secret is that the one who manufactures it will not have to answer a lot of questions. Keeping the drug open will invite a lot of curious questions. It can be tiring but when these inquiries are handled properly, they can actually illuminate and foster understanding and appreciation of the drugs. The negative consequence of bringing the drug into the open is that there may be businesspeople that would become interested in the drug and would use inappropriate means to have it. The ethical principle in my system that would be applicable in judging whether I should use the drug or not is the determination of whether the use of it will do me good or not. The drug has already been scientifically proven that its users felt happy, never violent, never depressed, always in a good mood, quick to laughter and, in general, of  a positive outlook.  

History of Brazil Essay Example for Free

History of Brazil Essay It is a matter of fact that Brazil is a country of contrasts. One can easily observe that the development of this nation is incredibly uneven. Taking into consideration their past experience, the Brazilians are seeing some very good times as a nation. Certainly, they have overcrowding problems. However, each country has its own difficulties and tries to overcome them. Thus, the diversity of this country and its amazing history has brought a â€Å"mixing bowl† of culture preparing a bright future for Brazil. It is widely known that four major groups make up the Brazilian population. They are the Portuguese, who colonized Brazil in the 16th century; Africans brought to the country as slaves; various European, Middle Eastern, and Asian immigrant peoples who have settled in Brazil since the mid-19th century; and indigenous people of Guarani and Tupi language. (Skidmore, 131) Brazil is the only Latin American nation that takes its language and culture mainly from Portugal. Intermarriage between indigenous people or slaves and the Portuguese was a common phenomenon. Despite the fact that the major European ethnic culture of Brazil was once Portuguese, waves of immigration have greatly contributed to a diverse ethnic and cultural heritage. Admiral Pedro Alvares Cabral claimed Brazilian territory for Portugal in 1500. The early explorers brought back a wood with them that produced a red dye, pau-brasil. This is where the land received its original name. Portugal began colonization in 1532 and made the area a royal colony in 1549. During the Napoleonic Wars, fearing the advancing French armies, King Joao VI left the country in 1808 and set up his court in Rio de Janeiro. He was brought home later in 1820 by a revolution, leaving his son as regent. When Portugal wanted to reduce Brazil to colonial status again, the prince declared Brazils independence on Sept. 7, 1822. Thus, he became Dom Pedro I, emperor of Brazil. Harassed by his Parliament, Pedro I resigned in 1831 in favor of his five-year-old son who became emperor Dom Pedro II in 1840. Emperor Pedro II ruled to 1889 when a federal republic was established as a result of a coup detat organized by Deodoro da Fonseca, marshal of the army. A year earlier, while Dom Pedro II was in Europe, the Regent Princess Isabel had abolished Slavery. Abreu, 311) Dom Pedro II was a popular monarch. Yet discontent grew up and, in 1889, he had to resign because of a military revolt. Although a republic was proclaimed at that time, Brazil was ruled by military dictatorships until a revolt allowed returning gradually to stability under civilian presidents. From 1889 to 1930, the government was a constitutional democr acy. The presidency was alternating between the dominant states of Sao Paulo and Minas Gerais. This period ended with a coup detat that placed a civilian, Getulio Vargas, in the presidency. He remained as a dictator until 1945. From 1945 to 1961, presidents of Brazil became Eurico Dutra, Vargas, Juscelino Kubitschek, and Janio Quadros. When Quadros abdicated in 1961, he was succeeded by Vice President Joao Goulart. (Abreu, 329) Goularts years in office were marked by high inflation, total economic failure, and the increasing influence of radical political parties. The armed forces alarmed by these developments organized a coup detat on March 31, 1964. The coup leaders chose Humberto Castello Branco a president, followed by Arthur da Costa e Silva (1967-69), Emilio Garrastazu Medici (1968-74), and then Ernesto Geisel (1974-79). All of them were senior army officers. Geisel began a liberalization that was carried further by his successor, General Joao Baptista de Oliveira Figueiredo (1979-85). In the last of a long series of military coups, General Joao Baptista de Oliveira Figueiredo became president in 1979. He pledged a return to democracy in 1985. Figueiredo not only allowed the return of politicians exiled or banned from political activity during the 1960s and 1970s, yet also permitted them to run for state and federal offices in 1982. (Alden, 284) However, at the same time, the Electoral College consisting of all members of Congress and also six delegates chosen from each state continued its activities of choosing the president. The election of Tancredo Neves on January 15, 1985, the first civilian president since 1964, brought a nationwide wave of optimism and activity. He was elected from the opposition Brazilian Democratic Movement Party (PMDB). (Alden, 287) However, when Neves died on April 21, Vice President Sarney became president. The latter was widely distrusted because he had previously been a devoted member of the military regimes political party. Collor de Mello won the election of late 1989 with 53% of the vote in the first direct presidential election in his 29 years. (Abreu, 378) Mello promised to lower the persistent hyperinflation by following the path of free-market economics. Having faced impeachment by Congress because of a corruption scandal in December 1992, Mello finally resigned. Vice President Itamar Franco took his place and assumed the presidency. Fernando Cardoso, a former finance minister, won the presidency in the October 1994 election having 54% of the vote. He took office on January 1, 1995. (Skidmore, 232) Cardoso has organized the disposal of bad government-owned monopolies in the electrical power, telecommunication, port, railway, mining, and banking industries. His timely proposals to Congress included constitutional amendments in order to open the Brazilian economy to greater foreign participation and to implement such sweeping reforms as social security, government administration, and taxation so as to reduce excessive public sector spending and considerably improve government efficiency. Alden, 298) During his short time in the office, Cardosos economic wisdom has made a measurable progress in overcoming Brazils poverty level. It is remarkable to observe how the Brazilian government makes certain efforts in order to address basic needs of its people such as education, distribution of meals, health care, and the promotion of childrens rights. Co-signed by the President of Brazil and 24 state governors, the Pact for the Children is intended to fully implement the constitutional and legal obligations providing for protection of children and adolescents. Several federal agencies supervise the execution of government programs for children and adolescents aimed to give Brazilian youth opportunities for a better life, shelter, education, and love. Thus, if not forgetting about the past mistakes and taking care of its nation, the Brazilians will surely come to the brightest future.

Essay --

10. In approximately 250 words, describe the status of woman in Egyptian society. Consider the following: Content: †¢ Jobs and tasks for woman In Egyptian society, women were given good standing status in the home and were customarily accommodated in mud brick homes, which were very useful in carrying out various works in the home such as: making the good and other stuff. The women frequently visited the marketplace to obtain items, which were obligatory for daily life in Ancient Egypt. †¢ Positions of responsibility for woman In Egyptian society, women were much on the higher side when associated to other empires around Ancient Egypt. In cooperation with gender which belonged to the same class that will be treated alike given the circumstances of Ancient Egypt society. Let along Women in Ancient Egypt were privileged to all the given rights, which men had. However, women had the right to manage property, which belonged to belonged to them, and if she wished, she could sell the property to buy and property she wanted without there being a barrier between them. More importantly many of the attributes to recognize the significant role of women in Ancient Egypt society †¢ Rights of marriage and divorce In Egyptian society, one of the many rights that belonged to women was whether they chose to marry. Nonetheless woman in Ancient Egypt were not vital marry. Countless women who chose to marry did so at quite a young age. †¢ Unique female leaders Many women in Ancient Egypt were ahead of own time. Hatshepsut was the first women to have held a rank as pharaoh and then began her rule as a pharaoh in 1,500 B.C.E. In addition, Hatshepsut also took care of her people as well as constructed place of worship to the gods in addition to o... ... Nile River. The inundating period persisted from June to September, depositing on the river's banks a coating of mineral-rich sediment perfect for rising harvests. Subsequently the floodwaters had retreated; Egypt expected little precipitation, so agronomists trusted on the Nile to water their harvests. Ancient Egyptians cultured emmer and barley, as well as more than a few other cereal iotas, all of which were used to create the two important food staples of bread and stout. Papyrus emergent on the banks of the Nile River and was used to produce paper. †¢ The succession of the pharaoh Nevertheless, the label of Pharaoh was inherited, consequently ideal that it was passed down from father to oldest son. More importantly, there were voluminous circumstances where the successor would be assassinated or even die by "chance" as well as have some else take his place.

Copyright Laws :: essays papers

Copyright Laws CHAPTER 1 Lennie and George are two friends during the depression. Lennie is a big guy that is not very intelligent. George is a medium size man that takes care of all the business. In the first chapter George and Lennie talk about getting a new job at a ranch. They want to save enough money to build their own ranch and â€Å"Live off tha fatt a the land.† They explain that they will have bunnies, alfalfa, and other goods to sell. CHAPTER 2 George and Lennie get the new job in chapter two. They meet the people they will be working with. Candy is an old man that has a dog. Slim is a respected man. Curley is the boss’s son who is small and used to box. Curley is also a jealous man who has a wife. Curley’s wife is known as a tart. She talks to all of the guys even when she shouldn’t. Crooks is a black man, the stable buck who isn’t aloud to be by the white people much. This chapter is mainly about George and Lennie meeting all of the new co-workers. CHAPTER 3 All of the guys on the ranch think Candy should get rid of his dog. The dog is old, stinky, and can’t even walk or eat. Slim tells Candy that the dog should be shot for his own good. Slim shoots the dog. Curley and Lennie get into a fight. Lennie doesn’t really understand why. Curly wants to fight. Curley is a jealous man and hates the fact that Lennie is bigger than him. Lennie blocks a punch from Curley and broke his hand. Lennie also got a new puppy in chapter three. Lennie spends as much time as he can with his new dog. CHAPTER 4 All of the men went to the whore house except for Lennie, Crooks, and Candy. When Lennie went to check on his puppy, he decided to talk to Crooks in his room. Crooks’ room was part of the stable. There were shoes, hay, horse reins, and horse shoes everywhere. Crooks didn’t really want Lennie to come inside, but he thought some company was better than none. Curley’s wife came to see what was up. Crooks told her to leave. Curley’s wife was mean and told Crooks that she would have him hung if he wasn’t nice to her. Copyright Laws :: essays papers Copyright Laws CHAPTER 1 Lennie and George are two friends during the depression. Lennie is a big guy that is not very intelligent. George is a medium size man that takes care of all the business. In the first chapter George and Lennie talk about getting a new job at a ranch. They want to save enough money to build their own ranch and â€Å"Live off tha fatt a the land.† They explain that they will have bunnies, alfalfa, and other goods to sell. CHAPTER 2 George and Lennie get the new job in chapter two. They meet the people they will be working with. Candy is an old man that has a dog. Slim is a respected man. Curley is the boss’s son who is small and used to box. Curley is also a jealous man who has a wife. Curley’s wife is known as a tart. She talks to all of the guys even when she shouldn’t. Crooks is a black man, the stable buck who isn’t aloud to be by the white people much. This chapter is mainly about George and Lennie meeting all of the new co-workers. CHAPTER 3 All of the guys on the ranch think Candy should get rid of his dog. The dog is old, stinky, and can’t even walk or eat. Slim tells Candy that the dog should be shot for his own good. Slim shoots the dog. Curley and Lennie get into a fight. Lennie doesn’t really understand why. Curly wants to fight. Curley is a jealous man and hates the fact that Lennie is bigger than him. Lennie blocks a punch from Curley and broke his hand. Lennie also got a new puppy in chapter three. Lennie spends as much time as he can with his new dog. CHAPTER 4 All of the men went to the whore house except for Lennie, Crooks, and Candy. When Lennie went to check on his puppy, he decided to talk to Crooks in his room. Crooks’ room was part of the stable. There were shoes, hay, horse reins, and horse shoes everywhere. Crooks didn’t really want Lennie to come inside, but he thought some company was better than none. Curley’s wife came to see what was up. Crooks told her to leave. Curley’s wife was mean and told Crooks that she would have him hung if he wasn’t nice to her.

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Being famous Isn't always as great as Its made out to be; It also comes with a variety of effects. Some examples of famous people who may be dealing with these effects are Rappers, Professional Athletes, and Talk Show Host. Famous people are always being harassed and bothered; whether it is by a fan or paparazzi, they never get a break. It is rear to be a celebrity and stay under the map; the radar is always on and its focused on them.The effects of being a famous actor are loss of privacy, schedule issues, and demeanor. To begin with, one of the main effects of being a famous actor is privacy. At no point in time are actors honestly every alone. Everywhere they go and everything they do Is always watched closely. For example, fans and paparazzi are always harassing famous celebrity. How much can an actor do with paparazzi trying to take pictures of every move they make? Along with the paparazzi you also have stalkers who play a big role In the loss of privacy celebrities lose.Stalke rs are everywhere you expect them not to be; they pop up Instantly almost like a virus and tart attacking you mentally and even sometimes even physically. Another thing that may affect famous actors privacy are rumors. The presses along with other huge media news broadcasting companies tend to like to spread rumors. Rumors can have a huge impact on anyone's life; especially famous people because all the rumors made about them expand in the public quickly.Whether the rumors are true or not, they still are an invasion of celebrities privacy. Famous actors being harassed and stalked can also put their own security in danger. For example, when paparazzi take pictures they are usually posted online or shown on television. If a stalker or a criminal Is familiar with the viewed location In the picture, they may now be able to harm or Intimidate that celebrity. Privacy Is a very Important part of everyone's life and being a famous actor can affect that.Incidentally, another effect of being a famous actor is scheduling. At most times in a professional acting career or any career at all in particular, people are constantly busy with work. Actors usually perform in movies, plays, or television shows. The process to do the following is not a short process, not in the slightest bit. With this being said, famous actors lose arsenal time for their selves. Actors are not able to do too much of what they want to do; instead they often have to do much of what's need to be done.Most people in the world are able to enjoy their selves throughout the week; celebrities can't because their schedules are so strict. Another effect of being a famous actor is incomplete work. With a schedule that is so filled pack with things that need to be done, It can be hard for some actors to complete certain task. For example, say an actor has a movie to shoot and It will take all day. Also say the actor has a meeting that's mandatory hat will make him or her lose out on money If they don't go, How will the famous meaning that some task may end incomplete.To change the topic, one final effect of being a famous actor is demeanor. Demeanor is very important if being a celebrity. Eyes everywhere are on celebrities at all times; some may not even know. Being a famous actor come with responsibility. Children, Teens, and Adults look towards celebrities as role models. Role models are usually someone that other look up too and want to be. If famous actors are not showing that they can be a role model, they ay lose fans instantly. Another effect that goes along with that is personal image.Personal image is also a huge situation that can affect a famous actor; image is the first thing that people Judge others off when they don't know them. If a celebrity image isn't good, in some cases people may think they aren't good. This can now affect the amount of fans they have and how much money they bring in. A famous persons attitude can also affect him or her in huge way. If a celebrity att itude is bad, people may tend to not like them. For example, if someone was to ask a actor for their autograph and they were disrespectful and said no, that actor would lose fans and money.In conclusion, being a famous actor comes with many effects. Once a actor reaches that high level of popularity their life is no longer only theirs. Everybody, everywhere, will know everything about you; its almost like the rolling camera never stops. It can sometimes be an amazing opportunity, but it still comes with its effects. Being a celebrity means giving up privacy, scheduling issues, and demeanor. Is becoming a famous actor really worth the effects it comes with? I believe the choice is up to the upcoming actors to make.

History of Nubian

History of Nubian Nubia is a place located alongside river Nile and extends from the southern part of Aswan to the town of Dabba. The bigger part of Nubia is in Sudan while a small potion is situated in Egypt. History clearly indicates the subdivision of Nubia into lower, upper and southern Nubia.Advertising We will write a custom essay sample on History of Nubian specifically for you for only $16.05 $11/page Learn More The lower and upper Nubia was the initial settlement place for Nubians. Nubia gained its fame from its perpetual activity of mining gold in addition to trading with ivory and ebony. It is among the earliest black civilization with its backbone mainly on natural wealth (Bulliet, 2001). Culture Kerma culture was the first empire to bring together the Nubian community and it is believed to evolve from Neolithic. At that time, 1750 BC, the kings were powerful enough to mobilize construction of walls and rich tombs filled with human sacrifices and possessions (Bull iet, 2001). Craftsmen in Kerma were excellent in pottery and metalwork. Egypt took over Nubia in 1500 BC to form the new kingdom of Egyptian colony. Their empire stretched from Euphrates to the 5th cataract of the Nile. After the conquest, monument such as Abu Simbel was built. When Egyptians left Nubia Kingdom of Kush came into being. This community adopted the Egyptian culture including their religion, music, language, and code of dressing. Assyrians invaded Kushite dynasty consequently pushing them down south to Meroe. At the present time, Muslims makes up the Nubian community. Their main median of communication is the Arabic language in addition to the Nubian language. Other distinctive features include the code of dressing, traditions and music. Jewelry was the most characterizing feature of Nubian culture. The jewelry was tailored to meet individual needs and specifications. Wedding festivity was a period to put on and display this jewelry. Nubian music in form of ‘shar ’ was performed during wedding ceremonies. Technology Monuments, artifacts and tombs signified advanced use of technology to attain civilization. The unique form of writing comprising of hieroglyphs and the advanced alphabetic scripts of 23 signs clearly indicates high level of technology (Bulliet, 2001). Another important factor to note is the pyramids which were constructed in Meroe (800 BC – 350 AD). The process of putting up these pyramids required great human intellect and skills. This forms a reason for the classification of tombs and pyramids as the wonders of the world.Advertising Looking for essay on ancient history? Let's see if we can help you! Get your first paper with 15% OFF Learn More The kingdom of Meroe was also described by a standing military force with sophisticated weapons. To support this point, military formation instituted by Candace of Meroe made Alexander the great to withdraw his force. Trade in the ancient time was t he lifestyle of many communities. Nubia acted as a trade corridor for people from tropical Africa and Egypt. Ebony and ivory from tropical Africa were channeled to Egypt via Nubia. This contributed to Nubian wealth. Diet Agriculture was the main source of food for Nubians. A variety of date palms was planted intensively. Export of these products acted as a source of income. Cereals and vegetables were cultivated for consumption purposes. After the construction of Aswan High Dam, irrigation was carried out extensively. Several governmental projects including the building of walls to stop land from flooding were done. On the contrary, summer periods were hard times in Nubia as sorghum and millet were consumed. There was a risk therefore of malnutrition simply because sorghum lacked vitamin B. Reference Bulliet, P. et al. (2001). Nubia, The Earth and Its Peoples. Boston: Houghton Mifflin Company.

Surrealism and Real in Spellbound and Un Chien Andalou Essays

Surrealism and Real in Spellbound and Un Chien Andalou Essays Surrealism and Real in Spellbound and Un Chien Andalou Paper Surrealism and Real in Spellbound and Un Chien Andalou Paper As Richardson’s rightly suggests, ‘if a film could be viewed as surrealist under certain conditions, this does not make this or that film a ‘surrealist’ one. In fact, there is no such thing as ‘surrealist’ film. There are only films made by surrealists, or films, which have an affinity or correspondence with surrealism’ (Richardson, 6). Both Un Chien Andalou and Spellbound should be regarded in the mentioned respect. Notwithstanding these film’s surrealist connotation, which are especially evident in Bunuel’s and Dali’s film, the first thing that links them to reality is formal techniques such editing, sequencing of images and events, structural elements, which unravel in real time, creating framework for interpretation. However, as Un Chien Andalou vividly exemplifies, at least formally surrealism also contradicts certain conventions in realist representation of reality. The latter is reflected in the absence of linear and logarithmic correlations and causal ties, recursive and programmable parallel sequences. By means of exposing inner structure of the movie, the symbolic unity of cultural content is often broken and the audience is left in front of grotesque and estranged reality (Richardson, 67). In comparison to traditional realist approach, Un Chien Andalou lacks clear temporal and spatial coherence and logic. The plot in conventional sense is absent from the film and its temporal fabric is broken into pieces and temporal leaps – the starting sequence ‘once upon a time’ inadequately narrates the image sequence of man’s sharpening the razor and then slitting the eye of his wife with it. The image of moon, being cut by the sharp object seems to create association with opened eye. The meaning of this scene, if interpreted in a strictly realist perspective is absent, as the episode is not explained and connoted in a coherent way. The absence of causal links between actions and implications create the atmosphere of nonsense. However, such an approach is not viable in this situation. The reality presented in this scene has dream logic, because it is filled with free associations and perceptive amplifications, which often have traumatic character. One of the basic purposes of such surrealist technique is increasing human perception, which should result in deep penetration in one’s own Unconscious and Real’s structure. Therefore, the purpose of such cinematic sequence is not providing with clear understanding of the logic, but amplifying experience of Real as it is in its ugliness and absurdity. The next scene is ‘eight years later’ and has not direct linkage with the previous sequence, which means that perception is understood by the authors as blind to the memory and time, as it is the only means for portraying the Real in its condition. This sequence is also filled with strange meanings and signs, which are seemingly impossible to interpret. The ‘lover’ wearing a nun’s clothing with a locked box on his neck. The same affect of fantasy is produced after the death of ‘lover’, when the ‘wife’ assembles the pieces of his clothing and he suddenly appears near the door, looking at his palm with the hole in it, from which many ants emerge. The scene has similarities with traditional method, used in horror films, however, its purposes are different, which is proved by the following episodes. The sequence is cut to the street, where an androgynous subject is poking at a cut hand, surrounded by the crowd, policemen etc. When a cut hand is finally placed by the police in ‘lover’ box, the blind androgynous figure is left alone in the middle of the street and then ran down by the car. The ‘lover’, being affected by this sudden death, becomes aggressive and tries to sexually offense the ‘wife’. As in the previous case, the analyzed sequence lacks clear logical links between its episodes. However, the episodes often seem to convey assertive meanings by themselves. The episode, when the ‘lover’ exposes his sadist essence after the death of blind figure, is obviously an allusion to Freudian concept of ‘will to death’, as deeply ties sexual desire with finite nature of human existence.

How to Read and Understand a Difficult Book or Chapter

How to Read and Understand a Difficult Book or Chapter Weve all encountered chapters or books that we just cant get into or we dont understand. There are lots of reasons for this: sometimes were required to read about a topic that is just plain boring, sometimes we try to read material that is written way above our current reading level, and sometimes we find that the writer is just plain bad at explaining things. It happens. If you find yourself reading an entire chapter or book several times without understanding it, try taking the following steps. Be sure to do steps 1 to 3 before you jump in to read the text. Difficulty: Hard Time Required: Differs by length of written material What You Need: A difficult book or passageNote paperPencilSticky note flagsQuiet room How to Do It 1. Read the introduction and reflect.  Any nonfiction article or book will have an introductory section that gives an overview of the main points. Read this first, then stop, think, and soak it in.Reason: All textbooks on a certain topic are not created equal! Every writer has a certain theme or point of view, and that will be introduced in your introduction. Its important to understand this theme or focus  because it will help you to recognize why certain examples or comments appear in your reading. 2. Look at the sub-headings. Most books or chapters will progress in some manner, whether they show a progression of time or an evolution of ideas. Look over the topics and try to find the pattern.Reason: Writers begin the writing process with an outline. The subheadings or subtitles you see in your text show you how the author started when organizing his/her thoughts. Subtitles show the overall subject broken down into smaller segments which are arranged in the most logical progression.   3. Read the summary and reflect.  Right after you read the introduction and subheadings, flip to the back of the chapter and read the summary.Reason: The summary  should re-state the points that were mentioned in the introduction. (If they dont, then this really is a difficult book to understand!) This reiteration of the main points may offer the material in more depth or from a different viewpoint. Read this section, then stop and soak it in. 4. Read the material. Now that youve had time to understand the points the author is trying to convey, youre more apt to recognize them when they come along. When you see a major point, flag it with a sticky note.   5. Take notes. Take notes and, if possible, make a brief outline as you read. Some people like to underline words or points in pencil. Only do this if you own the book. 6. Watch for lists. Always look for code words that tell you a list is coming. If you see a passage that says There were three major effects of this event, and they all impacted the political climate, or something similar, you can be sure there is a list following. The effects will be listed, but they may be separated by many paragraphs, pages, or chapters. Always find them and make note of them. 7. Look up words you dont understand. Dont be in a rush! Stop whenever you see a word that you cant immediately define in your own words.Reason: One word can indicate the entire tone or view of the piece. Dont try to guess the meaning. That can be dangerous! Make sure to look up the definition. 8. Keep on plugging through. If youre following the steps but you still dont seem to be soaking in the material, just keep reading. Youll surprise yourself. 9. Go back and hit the highlighted points. Once you get to the end of the piece, go back and review the notes youve made. Look over the important words, points, and lists.Reason: Repetition is the key to retaining information. 10. Review the introduction and summary. When you do, you may find that youve absorbed more than you realized. Tips Dont be hard on yourself. If this is hard for you, its probably just as hard for other students in your class.Dont try to read in a noisy environment. That might be ok under other circumstances, but its not a good idea when attempting difficult reading.Talk to others who are reading the same material.You can always join the homework forum and ask advice from others.Dont give up!

PERSONAL GOALS & REFLECTION ASSIGNMENT BUSINESS ETHICS Essay

PERSONAL GOALS & REFLECTION ASSIGNMENT BUSINESS ETHICS - Essay Example After evaluating my time audit sheet, I can say with confidence that I am maintaining the values that matter to me in my daily activities. The two most important influences in my life are religion, and education, and so it is appropriate that related activities (class, study, and prayer) occupy the majority of my days. I would not change any aspect of the way I fill these time periods, except for extending them if it were a realistic option. If I were conducting myself as a business with the values equivalent to those described in the memorandum, then the usage of my time is very efficient. It promotes the building of personal skills, spiritual health, social activity, and a very productive, happy life. Of course, some play time does creep into my days, but it is a necessary release of stress that surely contributes to my success during the day. I wouldnt expect my employees to spend their entire day without any fun. It would surely exhaust them in a short amount of time. If I were to make a single change to my routine, I would try to eliminate about an hour from my night of sleep. There has been evidence that seven hours or even less can be more than sufficient for some people, and the extra time could certainly be used for a more productive purpose. Though sleep is definitely important, I would be interested to see if I can be awake for longer without a major negative impact to daily

Consumer behavior Research Paper Example | Topics and Well Written Essays - 3000 words

Consumer behavior - Research Paper Example ect, the study of consumer behaviour is very essential for developing proper marketing strategies and appropriate product lines for automotive products. In general, there are many factors that influence consumer behaviours. Gilligan and Wilson have identified four primary factors that influence consumer behaviour and these are cultural, social, personal and psychological (Gilligan and Wilson, 2003, p.226). However, in automobile industry, the perceptions and preferences of consumers vary according to nature, unity, durability, safety, price and technology of automotive products. This paper will attempt to discuss consumer behaviour for non commercial automobile products like cars, bikes etc. The automobile products are consumer durable goods with high prices and a large variety of automobile products are available for different consumer groups. The primary objective of this paper is to analyse various consumer behaviour issues related to automobile products and to apply relevant consumer behaviour models in order to resolve them. This paper will deal with two main sections. The first section will present my own thoughts and opinions towards automobile products. The next section will attempt to identify the existing consumer behaviour issues for non-commercial automobile products. Various theories and models will then be applied to resolve these issues. Finally, the conclusion will reflect the major findings of this paper. Automobile industry has experienced the most frequent changes in the last decade. Development of technology and increase in consumer demand are two vital factors responsible for the growth of this industry. This has also led to the increase in competition among the automobile manufacturers. The companies are trying to introduce latest technologies for developing new motor cars that can match the expectations of existing customers. In this industry, the expectations of consumers vary significantly and the automobile companies are aiming to meet

E-Recruitment Overview Assignment Example | Topics and Well Written Essays - 3000 words

E-Recruitment Overview - Assignment Example It is seen that the Microsoft website is user friendly and has easy navigation. The website portrays the image that the company prefers to work with the jobseekers and only with the capabilities of their employees they have reached this position. Therefore they welcome competent people in their company. Though the Adidas website has a simple front page, yet it is very interactive. For those people who have fast connections, they can see a documentary in which a lady tells about all their expertise, lines of products, and consumer behaviours. For slower connections, there are certain pages that update the applicant about these facilities. Since it does not have an easy navigation, so the site cannot be considered user friendly. It has a moderate impact on the consumers; though people can have maximum knowledge from the website, yet it does not provide enough room to interact with the company. The KPMG website tells more about itself and its working, and hence gives a very rigid impact . It does not facilitate the candidates by showing the latest job openings on the front page and there is no link from where we can provide them with the feedback. This makes the site least friendly from the first page. Rolls Royce’s website has been the site that has given users the maximum chance of knowing the company in a glance. On the front page they have given the product lines, job hunt segregation and place to contact them. They have also used other small tabs through which the applicant can interact with the company. These elements make the navigation on the website much easy. It also shows that the company likes to facilitate the employees as much as possible. How the website/s attempt to set expectations related to... The assignment "E-Recruitment Overview" talks about the usage of the Internet in the placement of personnel and how new technologies change this sector. Due to efficient, fast and cost-effective nature of advertising, e-recruitment has taken an important role. Internationally the recruitment landscape has significantly changed in the recent past. Increasing skill shortages and low levels of unemployment in many domains have increased the competition in hiring the best person. Due to increased competition in the recruitment market, the organizations are tending to spend more efforts, time and resources in increasing the range of online and traditional advertising methods. The basic use of the internet and internet technology is transforming, and to some extent revolutionizing the ways in which human resource departments recruit job candidates. In the recent years, due to the internet, the recruitment process has undergone a dramatic transformation. In the current global economic recession, electronic recruitment helps to change the general perspective of the recruitment by offering highly needed cost-effective means of hiring. For the wide range of organizations throughout the world, e-recruitment has now become a significant part of the recruitment strategy. Not only this, it has progressively become a popular method for the job seekers in searching and applying for the jobs. This tool is more effective for the larger companies as the large firms are more known to the job seekers, thus the number of applicants is higher here.

Week 5 chapter 13-15 questions Assignment Example | Topics and Well Written Essays - 500 words

Week 5 chapter 13-15 questions - Assignment Example The origins of this concept is traced by to 1845 when journalist John OSullivan first used the United States Magazine, and Democratic Review, where he expressed the need to annex Texas to ensure the expansion of the American territorial boundaries. At the time, the journalist was reportedly protesting that England and France were meddling too much in the American affairs, something that he did not like. The term then grew in popularity and became a political word after that (Brands et al., 2012). The 1850s Compromise was the legislations that were proposed to help resolve the dispute that arose regarding slavery and the territorial boundaries just after the Mexican-American war. Some of the key provisions of the Compromise include the proposal that California be admitted as a free state to the Union. The second provision was that the part of Mexican that had not seceded be subdivided into two territories namely Utah and New Mexico and that these territories be free of slavery. Thirdly, the Compromise proposed complete abolition of the slave trade in the District of Colombia. Fourthly, the Compromise contained the Fugitive Slave Act, which required all Americans to assist the slaves that had returned. Lastly, the Compromise had a provision requiring Texas to give up parts of the western land which it had earlier claimed and received approximately $10 billion to help settle the national debt (Brands et al., 2012). Following the case of Dred Scott v. Sandford in which Scott sued for his freedom after the Missouri Compromise had prohibited slavery, Justice Taney ruled against Scott arguing that not American regardless of whether slave or free could be an American citizen. In fact, the judge continued to argue that Scott could still not have won even if he were to be a legal plaintiff. This ruling favored the sectionalism in the sense that it meant that slavery would still be practices in Missouri despite having been outlawed by the Compromise (Brands et al.,

International marketing of Safari motors Essay Example | Topics and Well Written Essays - 2250 words

International marketing of Safari motors - Essay Example Advancement in technology including communication efficiency and better international relations has contributed to the promotion of the international trade. Competition has however become a great challenge to the success of international trade but most companies are rising to the challenge. To achieve greater investments and better market opportunities in the international market, it is necessary that primary and secondary market research is done to ensure that information regarding the country is achieved. (Hollensen, 2004) This paper will therefore look into the requirements and plans needed for a company (Safari Ltd) to enter market successfully. The main issues that will be addressed by the paper will be; marketing across borders, risks and opportunities that the company will face and country analysis using SLEPT and size of the Chinese computer manufacture market. Safari motors is computer manufacturing company in the United States located in Texas. This company offers a wide variety of products including the manufacture, assembly, and maintenance of computers. The company has risen from being a retailer of used computers to become one of the improved companies in the sector. It boasts of a financial base of $ 120Million. The company has been for the past two years getting recognition and that it believes it has met the needs for the domestic market and now it needs to explore the international market. Changing nature of international environment There have been many changes on the way international market environments have been behaving especially in terms of diversity. The international marketing environments are usually done through the use of a STEP or PEST analysis procedure. (Hollensen, 2004) This analysis is worth because it determines whether the organization will be fairing well or not. For the purposes of this paper we shall use SLEPT marketing tool to analyze the Chinese market China China is the fastest economically-growing country in the Asia currently and is considered to be very developed come the next decade. Accompanying a rapidly growing economy is the increase in environmental pollution and this pollution is contributed by foreign companies which have heavily invested in China. Currently it is number two economy in Asia. (Lee and Carter, 2005) Ready market With an increase in the economic growth rate of the Chinese economy, most sectors will require computers to improve on its technology and service provision. The manufacturing sector in Chinese has grown bigger in the last few years demanding more usage of the computer software and hardware. The introduction of internet and other communication services such as the mobile phone sector, has taken the country by storm. With an increase in the need for internet access, comes an increase in the need for computer hardware and software. A study conducted by Coopers limited (UK) in China has shown a rise in the need for internet among the Chinese urban population especially the youth. (Lee and Carter, 2005) SLEPT analysis This is an analyzing tool which is used to examine a business environment it stands for social, legal, economic, and political. Social Forces within the society such as media, family and friends have affected the way an organization sells its products. Most of the customers in china believe that products made from America are of high quality or low quality or any other

Week 5 chapter 13-15 questions Assignment Example | Topics and Well Written Essays - 500 words

Week 5 chapter 13-15 questions - Assignment Example The origins of this concept is traced by to 1845 when journalist John OSullivan first used the United States Magazine, and Democratic Review, where he expressed the need to annex Texas to ensure the expansion of the American territorial boundaries. At the time, the journalist was reportedly protesting that England and France were meddling too much in the American affairs, something that he did not like. The term then grew in popularity and became a political word after that (Brands et al., 2012). The 1850s Compromise was the legislations that were proposed to help resolve the dispute that arose regarding slavery and the territorial boundaries just after the Mexican-American war. Some of the key provisions of the Compromise include the proposal that California be admitted as a free state to the Union. The second provision was that the part of Mexican that had not seceded be subdivided into two territories namely Utah and New Mexico and that these territories be free of slavery. Thirdly, the Compromise proposed complete abolition of the slave trade in the District of Colombia. Fourthly, the Compromise contained the Fugitive Slave Act, which required all Americans to assist the slaves that had returned. Lastly, the Compromise had a provision requiring Texas to give up parts of the western land which it had earlier claimed and received approximately $10 billion to help settle the national debt (Brands et al., 2012). Following the case of Dred Scott v. Sandford in which Scott sued for his freedom after the Missouri Compromise had prohibited slavery, Justice Taney ruled against Scott arguing that not American regardless of whether slave or free could be an American citizen. In fact, the judge continued to argue that Scott could still not have won even if he were to be a legal plaintiff. This ruling favored the sectionalism in the sense that it meant that slavery would still be practices in Missouri despite having been outlawed by the Compromise (Brands et al.,

Management in Middle East and North Africa Essay

Management in Middle East and North Africa - Essay Example This paper illustrates that the service industry is the fastest growing segment in the world and is the highest contributor to global output and employment among all the global business sectors. The increase in the level of privatization and urbanization along with more demand for final and intermediate consumer services have been the major drivers of the increasing importance of the global service sector. The availability of high quality of services is critical for the overall development of an economy. In the developed economies, the growth of both primary and secondary sectors is based on the growth of the main industries in the service sector like banking, trade, tourism, entertainment, education, insurance, hospitality, transport, retail etc. Both developed and developing countries are focusing on setting up a profitable and sustainable services sector in order to remain competitive in the evolving economic world. The changing demands and preferences of the global consumers have made service industry a necessity as well as an opportunity for different countries in order to develop their economies and position themselves as strong nations in the world. The increasing number and levels of cross-border transactions driven by globalization and liberalization have increased the demand for highly functional and beneficial service industries in different countries. The countries in the Middle East are competing on a global level through effective innovation and impotence given to the services sectors in these countries. The Middle Eastern countries like Qatar and Morocco have identified the importance of developing their service sectors to maintain sustainability in the evolving global world. The North African countries like Lebanon are seeing an increase in the investments and trade practices. The North African countries are developing after the advent of globalization. In this respect, they are focusing on improving the service sectors in the regions. Unlike ot her economies, Lebanon does not focus on a wide array of service industries. The country thrives mainly on commerce, tourism, and banking industries in the services sector. Morocco and Qatar, on the other hand, have multiple high performing industries in their services sector. International trade is a major service sector in Lebanon whereas other service industries like Information technology, retail, and utilities have not been established stringy in the country.

Funnist Incident in Classroom Essay Example for Free

Funnist Incident in Classroom Essay Alright so it was an ordinary day at school, and I was going to my locker in between fifth and sixth hour for the school-wide locker clean-out. I didnt have much to clean, I just emptied my locker of a few homework assignments that seemed to be hiding in the back. So I was done cleaning and I went to my sixth hour class, which really isnt much of a class as it is independent study. Its just me and these two kids from New Delhi who are in seventh grade and take pre-calculus. Well, the room I go to for sixth hour is a science room, so there are a few safety apparatuses to use, just in case: There is an eye wash station if you get chemicals in your eye and a ventilation wood if youre working with things that give off fumes. The last safety device is the safety shower, which is nothing more than a slight depression in the floor where there is a drain, a showerhead overhead, and this metal rod thing that you pull which releases an enormous gush of water. I think you use it if your clothes get on fire or if you accidentally spill chemicals on yourself. Anyhow, I was walking around the room, reading some science comics that were plastered to the wall. Well, I happened to slip on a rogue piece of cardboard that was lying on the floor, and I was spiraling out of control right towards the safety shower. I had no other choice, and plus I was a bit disoriented because I was spinning, so I grabbed on to the metal rod to stop myself from falling and WOOOOOSH A hundred gallons of cold water come down all over me! Im standing their soaking wet, not knowing what to do, and finally the teacher walks into the room and nearly drops his coffee. I was sent to the nurse and she gave me a big towel to wrap myself in. The nurse called my mom to let her know what happened, and she picked me up from school about twenty minutes later. The worst part wasnt me getting wet, oh no. When I activated the safety shower, the door to the classroom was open, and you could see me directly from the doorway! There was a whole crowd of people outside the adjacent classroom, and they started laughing ! I was so embarrassed!

Effects of Static and Dynamic Culture Conditions

Effects of Static and Dynamic Culture Conditions Tissue engineering has been investigating the properties of scaffolds and cell culture conditions for better cell attachment, viability and proliferation. This study compares the two cell culture conditions: Static and spinner flask / dynamic cell conditions over a period of 7 days on polyglycolyic acid. The scaffolds were statistically seeded by mouse dermal 3T3 fibroblast in static culture method and on other hand seeded scaffolds were transferred to spinner flask at approx.60 rpm in dynamic culture method. Significant improvement in cell viability was not observed in both the conditions after 7 days of culturing. The cells adhesion successfully took place and expressed cytoskeleton ÃŽÂ ²-actin in both the methods but achieving maximum distribution of cells on the scaffold in dynamic method. This study reports that static culture method could produce increase in cell number approximately six times more after 7 days of culture i.e. from 1.2 x 10à ¢Ã‚ Ã‚ µ ( ±0.1610à ¢Ã‚ Ã‚ µ) cells to 6.3 x10à ¢Ã‚ Ã‚ µ ( ±110à ¢Ã‚ Ã‚ µ) cells. Surprisingly, instead of enhancing the growth of 3T3 fibroblast cells in dynamic condition, they seems to be probably undergoing cell death/loss as reported by alamar blue, hoechst DNA assays, toludine blue and western blot. Overall, static condition favoured the cell adhesion, proliferation and ÃŽÂ ²-actin expression gradually with days and produced better reproducible data compared to dynamic condition. The techniques involved in dynamic culture method needs to be more carefully investigated and improved further to draw a strong conclusion. The aim of the study is to implement the principles of fundamental techniques in tissue engineering in culture method on the three dimensional polyglycolic acid (PGA) scaffolds seeded with 3T3 fibroblast. To compare and contrast the effects on cells in spinner flask or dynamic culture condition method with the static culture condition method by observing and analysing on factors like cell adhesion, distribution, proliferation, viability and expression of cytoskeleton after culturing in the same system for 7 days using alamar blue, hoechst 33258 DNA assays, toludine blue staining and western blot analysis. Tissue engineering is a multidisciplinary field which aims in developing new approaches for functional substitutes applicable in restoration of damaged or injured tissues. These substitutes are complex constructs of living cells, bioactive molecules and three dimensional porous scaffolds, which supports cell attachment, proliferation and differentiation. Therefore, its main objective to achieve in therapy is to form a living tissue from small population of mammalian cells. For this, the ideal tissue engineering strategy so far has remained to develop tissue by seeding the specific population of cells on three-dimensional constructs which not only provides a structural support to cell mass but also can effectively influence cell attachment, growth and differentiation either by incorporation of adhesion molecules or controlled release on bioactive molecules from the scaffold. After seeding of cells onto the 3-D scaffolds construct, the cells starts proliferating which results in deposi tion of extracellular matrix components and biodegradation of scaffolds. The latter makes the porous construct of scaffold more solid 3-D. Several other factors affect the 3D tissue growth including scaffold design, seeding method and the culture condition methods. Studies have reported that high degree of cell attachment to biocompatible and biodegradable particles, while avoiding aggregate formation can be achieved using poly co-glycolic acid (PGA) scaffold of 50-100mm spherical size fabricated by electro-spinning technique. This method provides reliable, reproducible and well-characterized PGA scaffold. The surface chemistry of the scaffold helps to determine the particle size, shape, morphology and distribution. Depending on the experiments, surface modifications are performed like formation of poly l-lactide-coglycolide (PLGA) via ring opening polymerisation and fibronectin coating to scaffolds. However, it is not the part of standard protocol. Depending on the size, the required cell density for maximum attachment may differ to obtained optimal cell attachment. The seeding is usually done using the cell suspension of a particular seeding density which allows for maximum dispersion of cells and well integration into the pores of the scaffolds. But for therapeutic purposes, however, this strategy is not sufficient enough to result in an overall improvement in conditions due to severe tissue damages. This can be overcome only by achieving relatively high degree of cell attachment to the micro-particle. Several factors and parameters influence the cell adhesion like the curvature of the particles, the particle material, the electrostatic charge of the particles, the surface motif of the particles, the interaction between cell and particles, the number of cells in the tissue culture and type of cell culture method implemented. It is also important to obtain homogenous cell adhesion to the scaffolds and avoiding clumping which will lead to the formation of cell-particle aggregates. This will prevent cells from appropriate uptake of nutrient from the media and hinder their subsequent growth. The mammalian cells are usually cultured in static or bioreactors condition. Here in this study, spinner flask system is employed which is also a kind of bioreactor as it provides the 3D environment. It is a flask provided with magnetic rod which keeps rotating constantly at specified speed. The nature of growing cells requires such dynamic condition to mimic the environment similar inside the body which gives sufficient nutrient supply, waste exchange, enhances ECM and gap junction formation, and cell-cell interaction. Most importantly it also helps maintain the cells differentiated in 3D which is needed for tissue formation. This characteristic is not maintained by static culture method. Hence, many 3D culture methods have been developed such as perfusion chambers, rotary vessels and commercial perfused bioreactors with improved capacity for mass transport of nutrients and waste product. They help in formation of relatively good quality of tissue by more enhanced cell differentiati on and also maintaining in that state. The static culture method used in this study, tissue culture plastic with seeded scaffolds remains untouched in the incubator. But with static culture, alternative shaking on a shaker and resting can also be employed to provide better supply of nutrient through media. The attachment characteristic of ECM proteins such as laminin, will also depend upon the cell type used. There are particular conditions needed to be optimised with each cell type. Most of the tissue engineering experiments uses 3T3 fibroblast only to optimise the cell culture condition where there is optimum cell adhesion is obtained before using the actual stem cell of interest. This is because, 3T3 fibroblast are known to easily attach to any surfaces due to presence of the high density of integrins on their cell surface. This will not only enhance the cell attachment but will also give maximum possible interaction with the particle. Cells that have spread on the particles exhibit a clear halo of cytoplasm surrounding their nucleus after the rearrangement of their actin skeleton. The attachment and spreading of cells to a substrate surface is often seen as a basic characteristic, but is, in fact, the initial process that subsequently influences and regulates cell growth, survival, migration and differentiation. In addition, cell-to-substrate interaction, mediated by integrins, also influence cell behaviour and signalling pathways leading to modifications in upstream and/or downstream cellular activity. Thus, a desirable substrate should allow sufficient and optimal cell attachment and spreading characteristics to occur. The 3T3 fibroblast media is used in which DMEM supplemented with 10% FCS enhances the cell attachment as the serum is highly protein rich and therefore, helps in cell in adhesion by supplying the ECM-proteins as well as nourishing them. Hence, the serum conditioning step is of critical importance in maintaining cells health and attachment in the culture. Materials and Methods Scaffold preparation and serum conditioning PGA FELT Scaffolds disc of 2mm x 10mm and 45mg/cc (TE005-50-10) was provided by Smith and Nephew research group, University of Nottingham. These non-culture scaffolds were then treated in 24 well tissue culture plastics (TCP) plates with 3T3 fibroblast media containing 500ml DMEM (Sigma G7513) supplemented with 10% FCS, 2mM L-glutamine and 1% AB/AM (Sigma A5955). All the scaffolds were statistically seeded on day 1 using non-culture treated well plates to encourage the cells of mouse dermal 3T3 fibroblast to adhere to the scaffolds at seeding density of 1x 10à ¢Ã‚ Ã‚ ¶cells/ml. 3T3 fibroblast cell suspension was added in TCP plates for all test and no cells in the blanks. The plates are then incubated overnight at 37 °C, 5%COà ¢Ã¢â‚¬Å¡Ã¢â‚¬Å¡ in air. The remaining cell suspension was then again resuspended in warm media to achieve 4 x 10à ¢Ã‚ Ã‚ ¶ cells/ml cell density and was stored at -20 °C till day 7 for Hoechst analysis. 3T3 fibroblast cells were used to seed the scaffolds to observe the cell viability, cell proliferation and ÃŽÂ ²-actin expression on day 1, when the cell culture condition was maintained static and day 7, after applying the two cell culture conditions (static dynamic) and maintaining for 7 days. Static culture In static culture condition, the seeded and non-seeded (blanks) scaffolds were kept in 1ml of warm 3T3 fibroblast media per well. These five culture plates were kept in incubator and cultured for 7 days at 37 °C, 5%COà ¢Ã¢â‚¬Å¡Ã¢â‚¬Å¡ in air. Spinner flask culture Two separate spinner flask filled with 50ml warm media each was used for seeded scaffolds and non-seeded (blanks) scaffolds. These flasks were kept in incubator by loosening the side arms and setting the magnetic stirrer approximately at 60rpm and cultured for 7 days at 37 °C, 5%COà ¢Ã¢â‚¬Å¡Ã¢â‚¬Å¡ in air. After following 7 days for culture conditions, the construct was then sacrificed for alamar blue, toludine blue and Hoechst analysis. Also, in addition cytoskeleton analysis using western blot was also carried out. The assessment of two culture methods, static and dynamic was done by producing five set of readings for static condition and four set of readings for dynamic condition where the experimental analysis were conducted using three replicates for test and blanks on day1 and day7 Alamar Blue Assay Staining was done using 10% alamar blue containing 1ml alamar blue (Serotec BUF012B) and 9ml HBSS without phenol red (Sigma H1387). The stain was kept in dark at 37 °C. The scaffold was transferred from seeding and culture conditions to new 24-TCP non-cultured plate with 1ml warm alamar blue after washing three times with PBS. The plates were then incubated at 37 °C, 5%COà ¢Ã¢â‚¬Å¡Ã¢â‚¬Å¡ for 1hr. The aliquots of 3 x 100 µl of alamar blue were transferred to 3 wells of 96 microtitre well plate including the blanks to measure fluorescence using plate reader (Ex530nm/Em590nm). The excess of alamar blue solution was aspirated and washed with 1ml sterile PBS. Toludine Blue Staining Scaffold for toludine blue staining was transferred to new non-culture treated 24well TCP plate and was treated with 1ml ice cold 95% (v/v) methanol in dHà ¢Ã¢â‚¬Å¡Ã¢â‚¬Å¡O for 5 mins after washing 3 times with 1ml warm PBS. Then fixative was discarded and scaffold was allowed to air dry at RT followed by treatment with 1ml aqueous 0.1% (w/v) toludine blue (Fisher chemicals BPE107-10) for 5 mins. The scaffold was again allowed to air dry at RT. Papain Digest and Hoechst 33258 DNA Assay The aliquot of cell pellet (4 x 10à ¢Ã‚ Ã‚ ¶ cells) prepared on day1 was treated with 1ml of papain solution (1.06mg/ml, pH 6.5) (Sigma P4762) followed by overnight incubation in waterbath at 60 °C. The serial dilutions of the papain digested cell pellet using hydrolysed papain solution as diluent was prepared for 4.0, 2.0, 1.0, 0.5, 0.25, 0.125, 0.0625, 0.0312 and 0 x 10à ¢Ã‚ Ã‚ ¶ cells. In the Hoechst 33258 DNA assay, the hydrolysed papain solution was used as blank. 5 µl of each aliquots + 70  µl Hoechst dilution buffer was added in triplicates in black 96-well plate including the blank. In each well, 100  µl Hoechst 33258 working solution (Sigma S6639) was also added and fluorescence was measured using plate reader (Ex 360nm/Em 460 nm) Western Blot 100 µl cold RIPA buffer (Sigma R0278) was added to the cell pellet (4 x 10à ¢Ã‚ Ã‚ ¶ cells) and the seeded scaffolds in eppendorf from day 1 and was kept on ice for 20 mins while vortexing every 5 mins. The cells were then snap freezed by placing it on dry ice for 1 min then 1-3 min at RT. The cells are resuspended by grating and spinning for 30 mins. The supernatant was used for western blot. 10 µl of molecular weight marker and each sample were loaded onto SDS polyacrylamide gel. The electrophoresis was carried out for 90 mins at 125V. After SDS-page electrophoresis, the filter paper, nitrocellulose and sponge were soak in transfer buffer (Invitrogen NP0006) with 20% (v/v) methanol. The assembled western blot tank was run for 1 hr at 25V. The immune-detection of protein ÃŽÂ ²-actin was performed using primary antibody anti-mouse ÃŽÂ ²-actin (Sigma A2006) and secondary antibody anti-mouse horse radish peroxidises (HRP) (Invitrogen G21234). Statistical analysis All the data obtained was calculated using MS-Excel spreadsheet and statistic Independent t-test and paired t-test analysis was performed using SPSS software. Results and Discussion Morphology of 3T3 fibroblast cells The cell of mouse dermal 3T3 fibroblast was obtained from T180 flask by trypsin digest method is shown in figure1. The flask was confluent enough (80%) and morphology of the cells seems to be intact and healthy. No sign of contamination was observed prior to seeding procedure. The morphology of 3T3 fibroblast cells are of flat and spindled shape. These cells form a well-characterised and established mesh like interconnected networks. This property of fibroblast cells make them ideal for cell attachment as they show anchorage property due to presence of integrins in ECM. Hence, using this cell type achieving maximum cell adhesion onto the scaffolds becomes ideal for this experiment. Effect on Cell viability in static and dynamic conditions The alamar blue assay was performed on the static and dynamic culture condition to observe its effect on 3T3 fibroblast cell viability is shown in figure 2a and 2b. The culture method employed aims to maintain or increase the cell viability when cultured for seven days. Under static condition (Fig 2a), only 1 group out of five showed significant increase in fluorescence whereas other two groups showed more or less no change in their fluorescence produced from day 1 to day 7. Also, on contrary two groups showed significant decrease in fluorescence on day 7 (Fig 2a). Hence, variable of results were obtained between groups. On the other hand, under dynamic condition, the cell produced more fluorescence on day 7 compared to day1 expect for one group. Therefore on an average, when mean of the static absorbance reading was taken, it showed that there is significant decrease in fluorescence (fig 2b). But in dynamic method, the increase in fluorescence day 7 (Fig 2b) was not significant enou gh. The 3-D construct of PGA scaffold provides with an environment to the cells where they remain viable in culture for several weeks. Moreover, they should successfully increase the cell viability after some days. However, our study reported that the cell viability decreased tremendously in cell seeded PGA scaffolds in static cell culture condition whereas the dynamic cell culture method was able to increase the cell viability over 7 days of culture. So, when comparing the two culture methods statistically showed difference in their overall effect on the viability of 3T3 fibroblast cells where dynamic condition is more but not effective enough. So, static condition did not improve the cell viability more than dynamic culture method. Effect on Cell distribution in PGA scaffolds The three-dimensional PGA scaffolds constructs enables the fibroblastic cells to adhere and to evenly distribute throughout the porous structure. To assess the uniform 3T3 fibroblast cell distribution in two different culture conditions, toludine blue staining was carried out on day 1 and day 7 on both conditions is shown in fig 3. Toludine blue stains cell dark blue within the 3-D construct. As observed in static condition, on day 1 the cells were successfully seeded onto the scaffold but compared to day 7 the cells are not evenly distributed throughout the scaffold. Also, the scaffolds were efficiently seeded on day1 under dynamic condition as the figure 3c shows cells stained with toludine blue. Surprisingly, on day 7 (Fig 3d), the scaffolds shows no cells at all. This means, that the 3T3 fibroblast cells under dynamic condition was eventually lost or died. The spinner flask culture system might have loose the cells by day 7 due to poor adhesion or vigorous rotation. The cell seed ed on day 1 was too low or error in carryout the technique. But this was observed with all the spinner flask condition system, where the success was 2 out of 4 groups (Supplementary data 3). However, this observation is more of debate because no other factors expect the condition itself could affect cell distribution as uniform distribution was achieved in all the five static condition (supplementary data 3) which used the same scaffolds and cell type. Effect on Cell proliferation in static and dynamic conditions 3T3 fibroblast was culture over 7 days in both conditions to also observe its effect on the cell proliferation are shown in figure 4 (a, b, c d). The standard curve obtained with known cell density for both static and dynamic of all the groups (fig 4a 4b) showed increase in cell density with increase in the fluorescence. The unknown cell density of the cells from these two culture methods on day 1 and day 7 was calculated and found that 2 out 4 groups from dynamic conditions had no cells in the culture on day 7. Therefore, only other two groups were considered to evaluate the cell number on day 1 and day 7. There was significant difference in cell density over 7 days of culture in static method (n=5)(fig 4c) and on contrast there was no significant difference in cell density in dynamic method (n=2)(fig 4c). Almost all the groups showed cell density on day 1 around 1 x10à ¢Ã‚ Ã‚ µ cells/ml which was the actual cell density seeded on day 1 (supplementary data 4). This shows that se eding performed on scaffold achieved effective adhesion of all the cells present. The mean cell number from 1.2 x 10à ¢Ã‚ Ã‚ µ ( ± 0.16 x 10à ¢Ã‚ Ã‚ µ) cells on day 1 increased to 6.3 x 10à ¢Ã‚ Ã‚ µ ( ± 1 x 10à ¢Ã‚ Ã‚ µ) cells on day 7 under static culture method (fig 4d). On the other hand, dynamic culture methods showed hardly any change in cell number over 7 days of culture i.e. 2.0 x 10à ¢Ã‚ Ã‚ µ ( ± 0.92 x 10à ¢Ã‚ Ã‚ µ) cells on day 1 to 2.5 x 10à ¢Ã‚ Ã‚ µ ( ± 1.96 x 10à ¢Ã‚ Ã‚ µ) cells on day 7 (fig 4d). Previous studies have reported using other cell types that they start proliferating within 24 hrs after seeding cells on scaffolds employing dynamic culture methods. Contradicting this, our results have shown that dynamic had really poor effect on cell proliferation. Moreover, 3T3 fibroblast cells were undergoing death during seven days of culture. Whereas, static culture method shows drastic increment in the cell number and thus supporting 3T3 fibroblast cell proliferation efficiently. The scaffolds used for alama r blue assay on day 1 were used for Hoechst DNA assay with same after washing step (same for day 7 scaffolds). The washing might have been too vigorous which resulted in cell loss. It could also be possible that cells are being aspirated off from the culture which gave poor or no cell proliferation. It should be also taken into account that the success rate with dynamic culture method on cell proliferation was null out of 4 demonstrations. Expression of Cytoskeleton For the analysis of expression of cytoskeleton ÃŽÂ ²-actin on 3T3 fibroblast in two different conditions was done by western blot as shown in figure 5. The cell pellet of density 4 x10à ¢Ã‚ Ã‚ ¶ cells/ml was loaded against the cells obtained on day1 and day 7 from static and spinner flask culture method. The density of ÃŽÂ ²-actin obtained from the cell pellet was maximum. The amount of ÃŽÂ ²-actin detected on day 1 was lower than day 7 in static culture condition. It was the opposite scenario with spinner flak method where day 7 had minimum amount of ÃŽÂ ²-actin compared to day1. In some cases of spinner flask method ÃŽÂ ²-actin was not even detected on day 7 (supplementary data 5). Hence, comparatively the expression of ÃŽÂ ²-actin was higher in static culture method. Perhaps, it could be because the cell could not proliferate much as expected. Also, the culture didnt have enough cells left to express ÃŽÂ ²-actin on western blot. The formation of ECM cytoskel eton was not shown to be supported by spinner flask method. Conclusion and future work The tissue engineering scaffolds constructs have been shown more effective on cells containing serum in spinner flask/dynamic culture method rather than in static culture method. But from our data, it shows that dynamic condition only favoured cell adhesion and distribution. It was also able to produce a small increment in cell viability unlike static culture method. Contradicting the other data, cells were virtually not detected on day 7 and so is the expression of ÃŽÂ ²-actin. Not only this, all the 4 demonstration failed to show that cell growth can be effectively supported in dynamic culture method. Three seeded scaffolds were kept in spinner flask together, where there is increased chance for it to come in contact with each other. Cells may get detached from the scaffolds as it might be loosely adhere to the scaffold. The continuous rotation of magnetic rod in the flask circulates the media to provide nutrients to cells more effectively then static. Despite of this fact, the cells were either undergoing cell death or dislodged from the scaffolds or may be aspirated off from the culture. The static culture method have been effective in 3T3 fibroblast adhesion on the construct after seeding and eventually could improve tremendous cell growth by showing increase in cell proliferation over a period of seven days in culture. However, better distribution and increased ÃŽÂ ²-actin expression could only be achieved by the static culture method after 7 days as the cells proliferated more. Moreover, the success rate for this method was more compared to dynamic and produced more reliable and reproducible data. Hence, it can be concluded that static culture method supported cell growth better then the dynamic culture method. It would be interesting to investigate the technique involved in dynamic culture method more carefully to produce reliable data where it could be compared with the static method to give better understanding of the environment cells require to grow in artificial ECM-like structure and culture media. Since, within the body the cells are continuously under the force by blood flow in 3D environment, it would be useful to derive cell culture growth better in dynamic condition with enhanced technique. It is strongly recommended to carry out further research in this area to conclude spinner flask methods effect on 3T3-fibroblast cells with more reliable data. Evaluation The practical session assessed my learning in the techniques and concepts involved in tissue engineering. The demonstration on different techniques to prepare scaffolds assessed my understanding better and was helpful to apply same in this practical session by evaluating the different parameters that can be influenced by the scaffold design alone. As earlier discussed troubleshoot, implementing the technique given in protocol helped to produce the good replicates and contamination free-blanks and controls. While working in the hood with the partner, things were discussed prior to carrying out the experiment and working space was kept ready which helped in managing the use of same equipments, solution and incubation time effectively to avoid any source of contamination. Also, the exchange of results and data between several groups also led to the exchange of ideas and different cause for their results. However, the exact reason for spinner flask method to not work out is still not cle ar as all the groups got same reading where cells were present onto the scaffolds during alamar blue assay on day1 and day 7 but eventually lost when subsequent assays were done for same day. Overall, the difference between the effects of two culture method was evaluated. Acknowledgement The efforts put in by the Paula Ellis is acknowledged was carryout the change of media and taking care for the samples throughout the practical session and also during weekends. Also, Dr. Felicity Rose for giving the guidance and helping with doubt regarding the techniques and protocol. The images and data taken from all other groups are acknowledged for sharing their data used in this report. The effort of the group member is also acknowledged for managing with the working protocol load effectively. Figures Figure1. 3T3 fibroblast cells in T180 flask (10X). The image shows morphology of 3T3 fibroblast prior the trypsin digest followed by static seeding. The image was taken using Nikon (Scale bar: 80 µm) Figure 2a. Alamar blue assay for all static (n=5) and for dynamic (n=4) culture methods on day1 and day 7. The graph shows fluorescence detected  ± SD for both the culture condition. The absorbance value of non-seeded scaffold (control, Ac) was subtracted from the absorbance value obtained for seeded scaffold (As) to optimise the calculated fluorescence i.e. As-Ac. This was done for all the static and dynamic culture methods data. The statistical analysis paired t-test was at 95% significance level was done using SPSS. The calculated data is provided in the supplementary data. Figure 2b. Alamar blue assay of static and dynamic condition on day 1 and day 7. The mean of all the values on day 1 and day 7 for static (n=5) as well as dynamic (n=4) was done. The graphs shows the mean of absorbance (O.D)  ± SD. The statistical analysis was performed using paired t-test and independent t-test at 95% significance level. DAY 1 DAY 7 Figure 3. Toludine blue assay. The toludine blue staining was performed on static culture condition on day 1 (a) and on day 7 (b). Similarly for dynamic culture condition on day 1 (c) and day 7 (d) was carried out. In (a) and (b) there is darker background staining but (c) shows proper stained 3T3 fibroblast cells. No cells staining can be detected in (d) (Scale bar: 100 µm). Figure 4a. Standard curve for all static condition using Hoechst 33258 DNA assay. The standard curve was plotted using the known concentration 4.0, 2.0, 1.0, 0.5, 0.25, 0.125, 0.0625, 0.0312 and 0 x 10à ¢Ã‚ Ã‚ ¶ (blank) of 3T3 fibroblast cells against the absorbance obtained. The blank was subtracted from the test reading to standardise the graph. The graph was produced using MS-Excel, to obtain the linear regression and linear equation for each group to calculate the cell density in static culture condition. Figure 4b. Standard curve for only two dynamic condition using Hoechst 33258 DNA assay. The standard curve was plotted using the known concentration of 4.0, 2.0, 1.0, 0.5, 0.25, 0.125, 0.0625, 0.0312 and 0 x 10à ¢Ã‚ Ã‚ ¶ (blank) of 3T3 fibroblast cells against the absorbance obtained. The blank was subtracted from the test reading to standardise the graph. The graph was produced using MS-Excel, to obtain the linear regression and linear equation to calculate the cell density in dynamic culture condition on day 1 and day 7. Figure 4 c. Hoechst 33258 DNA assay was carried out on all static (n=5) and dynamic (n=2) culture condition on day 1 and day 7. The cell density was calculated using the standard curve for its own respective group. The graph shows cell density (x 10à ¢Ã‚ Ã‚ µ cells/ml)  ± SD for static and dynamic condition. The calculation was performed on excel-sheet and statistical analysis of paired t-test was done using SPSS. Figure 4 d. Hoechst 33258 DNA assay. The unknown cell density calculated from standard curve was averaged (mean) for static (n=5) and dynamic (n=2) culture methods. The graph shows cell density (x 10à ¢Ã‚ Ã‚ µ cells/ml)  ± SD for static and dynamic condition. The calculation was performed on excel-sheet and statistical analysis of paired t-test and independent t-test was done were appropriate using SPSS. Figure 5. Western blot analysis of 3T3 fibroblast cell from static and dynamic on day 1 and day 7. The expression of ÃŽÂ ²-actin in both culture methods are analysed using the rainbow marker and compared with the actual pellet of 3T3 fibroblast to cells extracted from two different culture methods on different days.

Importance of Bernard in Arthur Millers Death of a Salesman :: Death Salesman essays

The Importance of Bernard in Death of a Salesman  Ã‚     Ã‚   All of the characters in the performance Death of a Salesman have special traits that are indicative of their personality and literary purpose in the piece. Each serves a particular purpose and symbolizes distinct goals, functions, or qualities. The author places every character in a specific location to contrast, or emphasize another character’s shortcomings, mistakes, or areas of strength.   For this purpose, Bernard, a character in Death of a Salesman, is placed next to Biff, the protagonist’s son. Biff, is lost in a world created by his dazed father, who instills in him a set of false values, and eventually becomes a failure in his early age. In spite of the fact that Bernard admires Biff and believes he is able to help him prosper, Biff is unable to listen. Bernard also interacts with the protagonist himself, again showing the same traits that are indicative of his character. Bernard, who is a successful student and later a successful attorney, is opposi te the characteristics Biff is taught makes a man great. Our first example of Bernard's character is his interaction with Biff is in Act I, when the reader infers Bernard is tutoring Biff: â€Å"Biff, Listen Biff, I heard Mr.Birnbaum say that if you don’t start studyin’ math he’s gonna flunk you and you won’t graduate. I heard him!" These initial statements, spoken by Bernard, are indicative to the reader of how helpful he tries to be to Biff. He is among the only characters with a sense of reality; the only character that tries to help Biff take concrete, analytical steps to helping him succeed. He understands the consequences of Biff’s actions, and tries to dissuade his directionless ambition towards a more solid goal. â€Å"He’s gotta study Uncle Willy. He’s got regents next week.† â€Å"Just because he printed University of Virginia on his sneakers doesn’t mean they’ve got to graduate him, Uncle Willy.† Once again, this illustrates Bernard is the one of the o nly characters in tune with reality. He cares for Biff and wants to see him graduate. This is why he is constantly pushing Biff to complete his work. As Bernard matures, he continues his modest, responsible attitude towards life. The protagonist himself is confronted with Bernard’s character, and comes to terms with the sudden insight his son is no where near as well off as Bernard, even though they were initially given the same opportunities.

what woen could have been :: essays research papers

What Women Could Have Been? At the start of the 1900’s women’s separation was seen at every turn. In fact it was a long road of change that many females faced and many tried to persevere. It was impossible for these women to break down these walls and barriers. For if they had accomplished female change in America the roles of females would have been much more different in society today. For these women I see a future full of opportunity, had women been successful in their push to vote, their need for education, and proving their worth during the war effort. If any or all of these events happened that the women were so adamant about seeing change in, then their lives would have been drastically enhanced with changed. I feel that women’s struggle for equality in today’s society comes out of the inability to vote. Throughout Susan Ware’s novel Letter to the World, voting is seen as catapult for fairness and equality. It is said that if women had the right to vote that they would have voice and be able to involve themselves on the same playing field as men in the political arena. The fellow First Lady Eleanor Roosevelt said it best when she stated that â€Å"Women must learn to play the games as men do†Ã¢â‚¬ ¦Ã¢â‚¬ Throwing mud from the outside won’t help.† If this message had successfully been seeded in the female publics mind and taken root then a true political movement would have taken place. In retrospect this would have given women the right to form groups, change laws, and become more then just the male society’s doormat they. Education is another aspect vital for women’s growth and if it had worked it would have almost entirely changed every facet of today’s work force. If the average women would have got involved in helping make education a top priority, then education for women would have been entirely possible. If there were big groups and rallies of women behind the education reform, than there would have been a gradual acceptance for education. Instead the reform was put into the hands of very few women such as Eleanor Roosevelt, Dorothy Thompson, and Margaret Mead. These women worked hard to push for their right to education in a world of male dominance and they would have succeeded had the rest of America got on board for the fight.

A charity appeal letter for Action Against Hunger

I am the director of a company called Action against Hunger which You may have heard of, I am writing to you to ask your generosity and kindness to those less fortunate than you. I am hoping that you can spare a small amount of time to read my letter, and consider making a donation. You may be thinking that this is just another junk letter asking for money for a worthless cause, but we need your help. Firstly, Action against hunger is an organisation that helps children and adults all around the world suffering from malnutrition Action against Hunger’s vision is of a world without hunger. A world in which all children and adults have sufficient food and water, equitable access to the resources that sustain life, and are able to attain these with dignity. Our mission is to save lives by eliminating hunger through the prevention, detection and treatment of malnutrition, especially during and after emergency situations of conflict, war and natural disaster. From crisis to sustainability, we tackle the underlying causes of malnutrition and its effects. By integrating our programmes with local and national systems we further ensure that short-term interventions become long-term solutions. As you can see, we do a lot of things for people that are less fortunate than us, and we need all the donation we can get to keep this going. Your support is immensely powerful. Regardless of the amount, it enables our field workers to save lives, feeding children on the brink of death, helping isolated and vulnerable communities, and providing families with clean and safe drinking water. You can change a person’s life. So why wait? Furthermore, the expected rains across large areas of the region (including north east Kenya and large parts of Somalia) have failed, leading to an severe food crisis. Thousands of families, including around 70,000 children, have been forced to flee the drought-stricken homes to survive, with many joining overcrowded refugee camps. What if you were in this position? And you were the one begging for food. They do need your help, or the worse could happen. No person was born to die. So help fight global hunger!

Admissions MBA

I can vividly remember how happy I am to do trade-ins with my friends and siblings when I am still a kid. Every time I like something which my friends or siblings have, I try to convey them to trade in to what I have. With small and simple talks, I able to make them compromise to what I have offered. Unknowingly, that simple gesture already foretold of what I would be like when I become an adult which is to do business.I am a graduate of computer science and hold a master’s degree in the aforementioned course but the interest that I have with MBA has never subsided. I am very much interested in MBA with concentration in Management.MBA or Master of Business Administration is everyone’s dream. It is an achievement for an individual to finish this master’s degree. I have a number of reasons why I want to do an MBA.It is basically for my self-improvement however I have five reasons that are on my top list. First thing is, I firmly believe that MBA program would allow me to have further studies that would enhance my knowledge and skills related to the field of my specialization; thus, it will get me updated to any recent buzz related to this field.Secondly, MBA offers a lot of opportunities especially we are in this modern and competitive world where everyone must has its own edge to cope the competition; thus it is an edge for me if I have an MBA. Thirdly, MBA ensures excellence and eminence business education that equips individuals to be competitive and perform tasks efficiently.The fourth one is it gives me more opportunities to be exposed to different subjects such as economics, international business, marketing, finance and etc. that I don’t learn in my previous studies and I have the chance to concentrate on my chosen area of specialization.The last one would be, MBA does not only equip me academically but it would change my perspectives in life through the rigid training and studies that I will be going through.

Muslim women today in Islam Research Paper Example | Topics and Well Written Essays - 2000 words

Muslim women today in Islam - Research Paper Example Muslim women in the modern age have a unique image which is sometimes full of misconceptions. We see them as the antithesis of modernity due to their cultural practices. Contrary to popular belief, the discrimination of women in many Muslim societies has nothing to do with them being Muslims. The idea that their religion encourages the poor status of these women is in reality an ignorance of their real place in Islam. The underlying philosophy of Islam with respect to women should therefore be delineated from the well-entrenched customs that have no attachment whatsoever from the Quranic principles. The treatment of women in patriarchal Muslim societies is not the true reflection of women in Islam. It is a mistake to misjudge some cultural practices as evidence of their status. These oppressions and injustices under the pretext of religion is nowhere near the ideal treatment they deserve. Women of today have rights in Islam, which although not thoroughly practiced across the globe, reveal their worth. While it is true that women is equally valuable as men, their difference in roles and functions have led others to believe that they differ in status. A woman is expected to obey her father and then eventually her husband, as this is regarded to be the Will of Allah (Pratt, 2005). A certain Quranic verse has led some to believe women’s inferiority: â€Å"Men are qawwamÃ… «n over women bima God faddala ba’d of them over others, and bima they spend of their own money†¦Ã¢â‚¬  (as cited in Mejia, 2007, p.10). This has been interpreted to mean that men as providers are physically and intellectually above women, thus justifying their authority. There is however evidence to the fact that the hierarchy of gender is not Islamic but was a cultural practice from elsewhere that was embraced my Muslims. Although a man and a woman are physically different, they are nonetheless

Cellular pathology Personal Statement Example | Topics and Well Written Essays - 500 words

Cellular pathology - Personal Statement Example ence I obtained from King Abdul Aziz Hospital, I have confidence that I possess the necessary skills that form the basis for a career in medical diagnostics specifically cellular pathology. I have knowledge about the process of collecting samples from the patients and protecting them from contamination before commencing diagnostic procedures. At King Abdul Aziz Hospital, I had a comprehensive exposure and practice on the process of slide preparation and observation through medical microscopes. The laboratory department at the hospital analyzed both slides prepared from tissues and cells derived from body fluids (Hanon-Fletcher & Maxwell 43). The experience at King Abdul Aziz hospital inspired me to pursue a degree in biomedical science. This saw me relocate to UK where I am almost actualizing my aspiration. In UK, I have been going through a thorough training on laboratory techniques. The practical sessions had particular interest in diagnostics through cellular analysis. A major area am currently well conversant with is cancer diagnosis and it forms my primary reason for the interest in pursuing cellular pathology at West of England University. In addition, the course equipped me on how to obtain and handle pathological specimens for cellular analysis. My desire in life is to develop diagnostic procedures that are efficient and fast. Diagnostic procedures that are in use currently are not favorable to patients due to long waits. I would like to see patients get their results instantly and accurately. Cellular pathology looks at the origin of a disease at the cell level as opposed to the symptomatic diagnosis employed by most laboratories today. In the pursuit of my desire, undertaking my cellular pathology studies at the University will help me acquire the knowledge and skills for actualizing my desire. Knowing the basis of a condition in time is a prerequisite for managing the disease. Cellular pathology is the way to go as it gives the most accurate results

Marketing Research Paper Example | Topics and Well Written Essays - 1250 words

Marketing - Research Paper Example Lenovo has manufacturing facilities in Shenzhen, Shanghai, Beijing, and Huiyang in China and at Pondicherry, India and a center at Whitsitt, North Carolina. It will not be out of place to note that Compaq and Hewlett Packard got merged in 2002. It was in 2004 that IBM sold off their PC business to a state Chinese company called Lenovo. The Compaq and IBM were two dominating brands in the market in the mid-1990s. Dell and HP emerged as two strong players in the PC market during late ‘90s while share of IBM went on reducing year after year. With that state of affairs and highly competitive market coupled with aggressive pricing strategies by many other manufacturers, IBM became a minority share holder in Lenovo. IBM held 18.4% share holding in Lenovo and Lenovo was granted rights to use IBM brand for 5 years as per the agreement; however, Lenovo got the product brand ‘ThinkPad’ from the IBM on a permanent basis. (IBM sells PC†¦2004) Lenovo is a state-owned compa ny having most of its operations in China barring a few installations in India and Mexico. India and Mexico operations are mostly for the local needs of that country. Before we embark upon the marketing plan for Lenovo, it would be worthwhile to do SWOT analysis. SWOT Analysis of Lenovo It will be appropriate to identify the Strengths, and Weaknesses of the company along with the opportunities that company can readily harness and issues that may pose threat to the company. Strengths: a. The company is well guided and supported by qualified and talented managerial pool of IBM. b. Lenovo PCs are endorsed by IBM brand ‘ThinkPad’ providing a confidence to the consumers in the market place. c. Manufacturing facilities being in China, the company enjoys not only the low cost of manufacturing but additional skilled manpower for its expansion project. d. Being a state-owned company, the company is well taken care of at the time of any change in local rules and regulations durin g normal and adverse market conditions. Weaknesses: a. The company may have trouble during natural disasters or calamities within the country to maintain normal production programs as most of its manufacturing facilities are in China. b. Being a state-owned company, decision making process may not be quick enough to thwart competition when it is need of an hour. Opportunities: a. The Personal computer market is at fast pace of growth; there is large untapped market locally and globally. Lenovo is well placed to take advantage of this burgeoning market. b. The new technologies are making deep inroads in the personal computer field, making it more user-friendly and thus expanding the market with new applications. Lenovo, being backed by IBM is positioned appropriately to exploit new developments in the field of hardware. c. IBM’s long known history of R&D in the field of mainframes, servers, and personal computers is likely to benefit Lenovo immensely in the coming days. The lo w-cost manufacturing facilities coupled with technological back up from technology giant forms a good synergy in the quality conscious and price sensitive markets. Threats: a. Lenovo is pitted against some of the well known brands of the companies such as Hewlett Packard, Dell, and Acer. Any mergers among them may certainly pose a big threat to Lenovo. b. Dell and HP are expanding their manufacturing operations in China. In the event of this, the cost advantage to Lenovo will get nullified in the future. Marketing Plan Product Strategy

Hollywood Mellodrama module Essay Example | Topics and Well Written Essays - 1500 words

Hollywood Mellodrama module - Essay Example ry workers widow with that of the bourgeois Communist couple in the former is more redundant than revealing; and the somewhat strained antic behavior of the characters in the latter virtually demolishes any sense of form. While that form keeps pretending that the film is melodrama, the action and acting often border on The Three Stooges. Still, Margit Carstensens remarkable incarnation of the ultimate, twisted groupie--to Kurt Raabs impotent poet who "only murders those he loves"--resonates with perverse gusto within the dynamics of the Fassbinder troupe. In yet another sense, style almost supplants content altogether in Chinese Roulette (1976), as a delirious use of camera movement and eccentrically composed shots become ends in themselves. If there is a point to the upper-class shenanigans in the film, it may be that the venalities to which parents expose their children will be visited upon them in turn. At the center of the film there is a "truth" game conducted by a crippled child (Andrea Schober) who seeks to humiliate her parents, particularly her mother (Carstensen), by associating her behavior with that of a commandant of a concentration camp. The films excessive stylization barely disguises its similarity to Fassbinders interrogation of his own mother in Germany in Autumn. Mise-en-scà ¨ne aside, Mother Kà ¼sters provides an important clue to Fassbinders politics, which were hardly either left or right. In the silent German film Mother Krausens Journey to Happiness (d. Piel Jutzin, 1929), on which Fassbinders is based, the mother bemoans her miserable life in the slums, and after her son is arrested, turns on the gas and escapes into the fantasized "happiness" of the title--although the film ends with a strong socialist message as her daughter marches with the masses to the "Internationale." Living in a time that has absorbed the failures of one ideology after another, Fassbinder debunks such idealized solutions and implies that the only way social